Sample loading

Automated Sample Loading for Reproducible HPLC

Sample introduction sets the tone for every chromatogram: get it right, and your calibration, quantitation, and audit trail stay trustworthy from the first vial to the last. Sykam’s fully automated sample loaders run unattended sequences of up to 120 positions with minimal carryover, programmable wash cycles per injection, and support for duplicate injections and variable injection volumes — so you can standardize complex methods without standing at the instrument.

Sykam HPLC sample loading

Mid-throughput autosampler

S 5250 — HPLC Sample Loader

Flexible racks, microplates, and firmware options for demanding analytical workflows

The S 5250 is built for labs that need a dependable, adaptable front end to HPLC: interchangeable sample racks and microtiter adapters pair with extensive firmware options so you can match the autosampler to your column chemistry, sample format, and regulatory expectations. Injection linearity and day-to-day repeatability are engineered in — and when your method calls for derivatization or variable aliquots, the platform is ready without compromise.

Specifications

Materials
Stainless steel, PEEK, PPS, or PVDF
Sample capacity
60 or 90 × 1.5 ml vials; up to 192 positions with microtiter plates
Injection volume
Programmable 0.1 – 999.9 µl

Applications

From pharmaceutical release testing to research methods that evolve weekly, the S 5250 fits wherever robust, traceable liquid handling is non-negotiable — including workflows with offline or online derivatization and multi-format sample submission.

Sykam S 5250 HPLC sample loader

High-throughput autosampler

S 5300 — HPLC Sample Loader

Extended vial capacity with the same precision and software depth

When queue length grows but data quality cannot slip, the S 5300 steps up: the same reproducibility, linearity, and derivatization flexibility as the S 5250, with a larger 1.5 ml vial tray for high-sample-count campaigns. Configure racks and plate adapters to mirror your LIMS submission pattern, then let firmware-managed wash profiles keep cross-contamination under control across long overnight batches.

Specifications

Materials
Stainless steel, PEEK, PPS, or PVDF
Sample capacity
120 × 1.5 ml vials; up to 192 positions with microtiter plates
Injection volume
Programmable 0.1 – 999.9 µl

Applications

Ideal for high-throughput QC, stability studies, and screening labs that need one autosampler platform to span routine quantitation, method tweaks, and plate-based sample sets — without sacrificing injection fidelity.

Sykam S 5300 HPLC sample loader

Need help choosing rack layouts, wash strategies, or integration with your stack? Contact us — we will align sample loading with your throughput targets and compliance framework.